2022年EPG厂家培训通知

 2022年EPG厂家培训

时间：12.11-12.13，地点：墨尔本（澳大利亚）

当地负责人：

Piotr Trebicki & Isabel Valanzuela（奥地利）

项目负责人：

Freddy Tjallingii（荷兰），Elisa Garzo & Alberto Fereres（西班牙）

尊敬的同行：

什么是EPG（刺探电位图）技术？

      将植物和昆虫（大多是同翅目昆虫）分别通过植物电极和昆虫电极连接，构成连通电路的一部分；植物电极插入植物生长的土壤中，而昆虫电极是通过银胶将一根非常细的金线粘在昆虫的背板部位而形成。当昆虫借助刺吸式口器取食植物时，由于口器刺入植物组织的部位不同，以及昆虫的活动（机体活动、吸食或流涎），导致连通电路的电势差变化，即波形的波动。通常情况下，可以一次记录持续8小时的8个重复实验（可能还有更多的实验配置方法）

     EPG常用于研究植物与昆虫之间的相互作用，包括植物抗性、宿主植物识别、植物病原体传播、植物防御的电生理方面等。

课程内容：包括讲座（上午）和实操（下午），在法拉第笼中使用、昆虫和植物的布线安装、监测记录（数据采集）、信号分析、数据处理和统计分析方向。

参会人数有限：由于设备和设施的限制，参会者Z多16人，若有更多人注册，将会使用候补名单。

会议费：

500澳元（约人民币2500元）

参会方式：回复邮件如下或直接回复当地负责人

Piotr.trebicki@agriculture.vic.gov.au

Isabel.valenzuela-gonzalez@agriculture.vic.gov.au

如想了解更多请联系www.epgsystems.eu,  www.hpis2020.com

EPG培训课程日常安排：epgsystems.eu

附英文通知：

HPIS 2022 satellite EPG training course

December 11-13, Melbourne, Australia

Local Organizers:

Piotr Trebicki & Isabel Valanzuela (Australia)

Program organizers:

Freddy Tjallingii (Netherlands), Elisa Garzo & Alberto Fereres (Spain)

Dear colleague,

What is the EPG (Electrical Penetration Graphs) technique?

A plant and a (mostly) Homopteran insect are made part of an electrical circuit by using a soil electrode for the plant and gluing a very thin gold wire to insect’s dorsum. Upon plant penetration by the insect, electrically caused waveforms indicate tissue positions of the stylet tips and insect activities (mechanical movements, ingestion, or salivation). Typically, 8 replicates can be recorded for periods up to 6-8h (more experimental configurations are possible).

EPG recording is commonly used to study plant-insect interactions as involved plant resistance, host plant recognition, plant pathogen transmission,  electrophysiological aspects of plant defenses, etc.

Course program: see attachment. This includes lectures (morning sessions) of and practical training (afternoon sessions) in Faraday case use, wiring and mounting insects and plants, monitored recording (data acquisition), signal analysis, data processing and directions for statistical analysis.

Number of participants limit. Because of equipment and facilities limitations there will be a maximum of 16 participants. When more people sign up a waiting list will be used.

Fees. 

Participants pay a fee of AUS $500

Registration:

Please reply to this e-mail or to one of the local organizers

Piotr.trebicki@agriculture.vic.gov.au

Isabel.valenzuela-gonzalez@agriculture.vic.gov.au

For more formation please go to www.epgsystems.eu,  www.hpis2020.com

Best regards,

Freddy Tjallingii