试剂盒 5HT S Q014-00 说明书

文档简介GroundworkBiotechnologyDiagnosticateLtd.1.GBDINTENDEDUSEThisGBD5HTELISAkitisintendedLaboratoryforResearchuseonlyandisnotforuseindiagnosticortherapeuticprocedures.TheStopSolutionchangesthecolorfrombluetoyellowandtheintensityofthecolorismeasuredat450nmusingaspectrophotometer.Inordertomeasuretheconcentrationof5HTinthesample,this5HTELISAKitincludesasetofcalibrationstandards.ThecalibrationstandardsareassayedatthesametimeasthesamplesandallowtheoperatortoproduceastandardcurveofOpticalDensityversus5HTconcentration.Theconcentrationof5HTinthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.2.GBDREAGENTSPROVIDEDAllreagentsprovidedarestoredat2-8°C.Refertotheexpirationdateonthelabel.1.GBD-586MICROTITERPLATE96wells2.GBD-516ENZYMECONJUGATE6.0mL1vial3.GBD-819STANDARD.I0ng/ml1.0mL1vial4.GBD-829STANDARD.II25ng/ml1.0mL1vial5.GBD-839STANDARD.III50ng/ml1.0mL1vial6.GBD-849STANDARD.IV100ng/ml1.0mL1vial7.GBD-859STANDARD.V250ng/ml1.0mL1vial8.GBD-869STANDARD.VI500ng/ml1.0mL1vial9.GBD-526SUBSTRATEA6.0mL1vial10.GBD-536SUBSTRATEB6.0mL1vial11.GBD-546STOPSOLUTION6.0mL1vial12.GBD-556WASHSOLUTIONx10010mL1vial3.GBDMATERIALSREQUIREDBUTNOTSUPPLIED1.Microplatereadercapableofmeasuringabsorbanceat450nm.2.Precisionpipettestodeliver2mlto1mlvolumes.3.Singleormulti-channelprecisionpipetteswithdisposabletips:10-100Land50-200Lforrunningtheassay.4.Adjustable10ml-100mlpipettesforreagentpreparation.5.100mland1litergraduatedcylinders.6.Calibratedadjustableprecisionpipettes,preferablywithdisposableplastictips.（Amanifoldmulti-channelpipetteis