Investigation of the Hydration of Nonfou领 Material Poly（ethylene glycol） by Low-Field Nuclear Mag

文档简介Thestrongsurfacehydrationlayerofnonfou领materialsplaysakeyroleintheirresistancetononspecificproteinadsorption.Poly（ethyleneglycol）（PEG）isaneffectiveexampleofmaterialsthatcanresistnonspecificproteinadsorptionandcelladhesion.Thus,thestronginteractionbetweenwatermoleculesandPEGwasinvestigatedthrougheachT2componentinwater/PEGmixturesusingmultiexponentialinversionofT2relaxationtimemeasuredbytheCarrPurcellMeiboomGill（CPMG）sequenceoflow-fieldnuclearmagneticresonance（LF-NMR）.Resultsshowthataboutonewatermoleculeistightlyboundwithoneethyleneglycol（EG）unit,andadditionalwatermoleculesover1:1ratiomainlyswellthePEGmatrixandarenottightlyboundwithPEG.Thisresultwasalsosupportedbytheendothermicbehaviorofwater/PEGmixturesmeasuredbydifferentialscanningcalorimetry（DSC）.Itisbelievedthatthemethoddevelopedcouldbealsoappliedtoinvestigatevariousinteractionsbetweenmacromoleculesandothersmallmoleculeswithoutusingdeuteriumsamples,whichmightopenanovelroutetoquantitativelymeasureguesthostinteractionsinthefuture.