pRevTRE说明书

文档简介Clontech四环素调控系统pRevTRE载体pRevTRE型号载体名称出品公司载体用途VSC0468pRevTREClontech四环素调控系统产品参数:Promotor:LTRSize:6500SelectableMarker:HygromycinConstitutive:InducibleViral/Non-Viral:Retroviral载体抗性:氨苄青霉素（Ampicillin）载体描述:pRevTREisaretroviralTetresponsevectorthatexpressesageneofinterestfromtheTet-responseelement（TRE）.ThisvectorisderivedfrompLNCX,aretroviralvectorcreatedusingelementsofMoloneymurineleukemiavirus（MoMuLV）andMoloneymurinesarcomavirus（MoMuSV）asdescribed.TheTREcontainssevendirectrepeatsofthetetOoperatorsequence,upstreamofaminimalCMVpromoter,whichcanbeboundbythetTAandrtTAtransactivators.The5'viralLTRcontrolsexpressionofthetranscriptthatcontainsY+（theextendedviralpackagingsignal）andthehygromycinresistance（Hygr）geneforantibioticselectioninmammaliancells.TheTREisderivedfromvectorsdescribedpreviously.pRevTREalsoincludestheE.coliAmprgeneforantibioticselectioninbacteria.　ThecompleteRevTet-OffandRevTet-OnSystemsalsoincludethecontrolvectorpRevTRE-Luc,whichwasconstructedbycloningthefireflyluciferasegeneintotheHindIII/ClaIsitesintheMCSofpRevTRE.质粒图谱: