pSilencer 2.1-U6 hygro载体说明书

文档简介pSilencer2.1-U6hygro载体描述:ThisAmbionvectorisfortheexpressionofsiRNA.Ithasanantibioticresistancegene（hygromycin）toenableselectionoftransfectedcellsandfeaturesthehumanRNApolymeraseIIIpromoter（U6）.Sufficientreagentsareprovidedfor20reactions.SelecttransfectedcellstoenrichthepopulationofcellsexpressingyoursiRNAEliminatetheneedtosynthesizeRNAoligonucleotidesforRNAiexperimentsSuppliedlinearizedandreadyforligationCompensateforLowTransfectionEfficienciesandPerformLong-TermStudiesTheuseofmammaliansiRNAexpressionvectorswithantibioticselectablemarkersconveysmanybenefits.Selectablemarkerscanhelpcompensateforpoorplasmidtransfectionefficienciesseenwithsomecelllines.Inthesecases,onlyafractionofthetransfectedcellsexpressthesiRNA,andreductionintargetgeneexpressionwithevenapotentsiRNAcanbedifficulttodetect.Useofaselectablemarkerandtransientantibioticselectionpermits.质粒图谱:上海起福生物科技有限公司联系人：杨建辉400电话：4006551678办公电话：021-50724187传真：021-50724961*8006手机：15921799099企业QQ:4006551678网址:http://www.qfbio.com/地址：上海市浦东新区绣川路561号1102室