pUB6/V5 His A说明书

文档简介pUB6/V5HisA型号载体名称出品公司载体用途VPI0022pUB6/V5HisAInvitrogen哺乳动物表达载体产品参数:KeyFunction：ConstitutiveExpressionMammalianSelection：BlasticidinPromoter：UbCVectorType：pUB6CloningMethod：RestrictionEnzyme/MCSProteinTagorFusion：V5EpitopeTag，HisTag（6xHis）ConstitutiveorInducibleSystem：Constitutive载体抗性:氨苄青霉素（Ampicillin）载体描述:pUB6/V5-HisA,B,andCare5.5kbvectorsdesignedforoverproductionofrecombinantproteinsinmammaliancelllines.Featuresofthevectorsallowpurificationanddetectionofexpressedproteins.High-levelstableandtransientexpressioncanbecarriedoutinmostmammaliancells.Thevectorscontainthefollowingelements:HumanubiquitinCpromoter（hUbC）forhigh-levelexpressionacrossabroadrangeofspeciesandcelltypes（Schorppetal.,1996;Wulffetal.,1990）.Threereadingframestofacilitatein-framecloningwithaC-terminalpeptideencodingtheV5epitopeandapolyhistidine（6×His）metal-bindingtag.Blasticidinresistancegene（bsd）forselectionofstablecelllines.EpisomalreplicationincelllinesthatarelatentlyinfectedwithSV40orthatexpresstheSV40largeTantigen（e.g.COS-1,COS-7）.Thecontrolplasmid,pUB6/V5-His/lacZ,isincludedforuseasapositivecontrolfortransfection,expression,anddetectioninthecelllineofchoice.质粒图谱:上海起福生物科技有限公司联系人：杨建辉400电话：4006551678办公电话：021-50724187传真：021-50724961*8006手机：15921799099企业QQ:4006551678网址:http://www.qfbio.com/地址：上海市浦东新区绣川路561号1102室