pCMV-Tag 2B载体说明书

文档简介型号载体名称出品公司载体用途价格VPS0040pCMV-Tag2BStratagene哺乳动物表达载体800产品参数:KeyFunction：ConstitutiveExpressionMammalianSelection：Neo，G418Promoter：CMVCloningMethod：RestrictionEnzyme/MCSConstitutiveorInducibleSystem：Constitutive载体抗性:卡那霉素（Kanamycin）载体描述:Epitopetagsprovideamethodtolocalizegeneproductsinlivingcells,identifyassociatedproteins,trackthemovementoftaggedproteinswithinacell,andcharacterizenewgeneswithoutcreatingprotein-specificantibodies.ThepCMV-Tag1vectorcontainsboththesyntheticFLAGepitopellllandthec-mycepitopefromthehumanc-mycgene.ThepCMV-Tag1vectorallowsproductionoffusionproteinsinavarietyofconformations.ThepCMV-Tag2-5vectorsalloweitherc-mycorFLAGepitopetaggingateithertheC-orN-terminus.EachpCMV-Tag2-5vectorissuppliedwithallthreereadingframesforeasycloningandexpression.ThesmallsizeoftheFLAG（eightaminoacidslong）andc-mycepitopetags（tenaminoacidslong）decreasesthepossibilityofinterferencewiththetaggedprotein.TheFLAGandc-mycepitopesarerecognizedbytheanti-c-mycandanti-FLAGantibodies,whichcanbeusedtocharacterizethetargetprotein.ThepCMV-Tagvectorsallowhigh-levelexpressionoftaggedproteinsinmammaliancells.Expressionisdrivenbythehumancytomegalovirus（CMV）immediateearlypromoter.TheCMVpromoterprovidesconstitutiveexpressionofclonedgenesinawidevarietyofcelllines.ThetranslationalstartsequenceusedinthepCMV-Tag1,pCMV-Tag2andpCMV-Tag3vectorsisa10-baseKozakconsensussequenceofCGG（A/G）CCATGG.pCMV-Tag4andpCMV-Tag5vectorsdonotcontainatranslationstartsequence.StablecloneselectionismadepossiblewithG418bythepresenceoftheneomycin-resistancegene.ThepCMV-Tagvectorsareonly4.3kb,allowingeasycloningandvectormanipulations.Thissmallsizeisduetothesingleantibioticselectionmarkerusedbybothprokaryoticandmammaliancells.Theneomycin-resistancegeneprovidesstableselectioninmammaliancellswithG418drivenbytheSV40promoterandkanamycinselectioninE.colicellsdrivenbythebla（-lactamase）promoter.质粒图谱:上海起福生物科技有限公司联系人：杨建辉400电话：4006551678办公电话：021-50724187传真：021-50724961*8006手机：15921799099企业QQ:4006551678网址:http://www.qfbio.com/地址：上海市浦东新区绣川路561号1102室