2. I think the best way after removal of the Fmoc- is to separate your dipeptide (a zwitterion) to aqueous phase and wash away the Fmoc-piperidine adduct and piperidine.
3. Then saturate your aqueous layer with NaCl and push your didpeptide to organic layer before further purification.calpain(站内联系TA)用20%哌啶/DMF溶液脱保护,脱除后蒸去DMF洗涤就好mylong872(站内联系TA)去保护基的混合物用乙腈/5%氨水就可以将产物和Fmoc分开