叶绿素荧光系统成像仪——PAM-WATCH

功能强大、操作简便、文献众多的叶绿素荧光成像系统

突变株快速筛选的强大工具

主要功能

• 成像功能：对Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等参数进行成像分析。测定调节性能量耗散Y(NPQ)，反映植物光保护能力，测定非调节性能量耗散Y(NO)，反映植物光损伤程度。

• 程序测量功能：可程序测量荧光诱导曲线、快速光曲线和暗弛豫，也可手动测量；在测量过程中能自动分析所有荧光参数的变化趋势

• AOI功能：可在测量前或测量后任意选择感兴趣的区域（AOI），程序将自动对选择的AOI的数据进行变化趋势分析，并在报告文件中显示相关AOI的数据。所有报告文件中显示的数据都可导出到EXCEL文件中。

• 成像异质性分析功能：对任意参数任意时间的成像，可在图像上任意选取两点，软件自动对两点间的数据进行横向异质性分析，并可导出到EXCEL文件中。

• 成像数据范围分析功能：对任意参数任意时间的成像，可分析任意两个荧光数值之间有多少个像素点，多少面积（cm2）。

• 突变株筛选功能：可跟据成像结果快速筛选光合、产氢/油、抗逆（抗盐、抗旱、抗病等）等突变株。

• 微藻毒理研究功能：可同时测量96个微藻样品（对照和处理组）的光合活性，软件自动给出处理组样品相对于对照组的光合YZ百分比。

• 吸光系数测量功能：快速测量叶片的吸光系数。吸光系数测量光源： 16个红光（650 nm）和16个近红外（780 nm）LED，用于测量植物叶片或藻类样品PAR吸光系数。

• 监测功能：可实时监控箱内环境状态、机器状态监控区显示目前程序及环境状态

• 环境仿真功能：可程序化多组程序功能 (可多至6组)，可个别设定执行天数，可设备单日环境参数（间隔可至分钟），程序化环境参数

• 项目管理功能：预约存多次测量的植物影像及参数，延时（缩时）摄影功能

• 数据分析功能：可导出照片文件，可导出叶绿素荧光参数Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等

系统设计

• 可单独作为叶绿素荧光仪使用，方便带到野外田间测量和成像；也可配置箱体式结构，容量300L，能同时满足植物培养和叶绿素荧光成像的功能。箱体内设有栽培区，可分2层、3层、4层等，调节高度进行培养和测量。箱体内有特殊消光图层，避免反光造成的测量干扰。

• 系统配置触摸式高清显示屏（24寸），方便操作，支持1670万色彩和FHD高清画质。

• 系统处理器：I7处理器，16G缓存，512固态硬盘SSD，满足快速数据处理和存储要求。

• 供电：室内使用220V，50Hz交流电，荧光仪单独使用时，内置可充电锂电池供电，满足4小时的野外测量

箱体环境控制（光照、温度等）

• 温度环控条件：15-40°C ±5°C（关灯）

• 光照系统：采用四合一LED光盘，白光5000k ±500k、红光660nm±10nm、蓝450nm±10nm、红外光730nm±10nm，光盘面积29×40cm，四种光源可独立自主控制，光质强度可达PPFD ≧700µmols-1m-2@15cm，光照时间控制可程序化设定，设定时间可至1min，可仿真日出至日落光照变化。

• 控制系统：PID微电脑控制，可设定上昼夜循环程序

• 可选配二氧化碳和湿度控制单元

成像技术参数

Maxi探头

• 荧光测量光源： 44个蓝色LED，450 nm，测量光强度0.5 μmol m-2 s-1PAR，ZD光化光强度1900 μmol m-2 s-1PAR，饱和脉冲强度4000 μmol m-2 s-1PAR

• 吸光系数测量光源：16个红光（660 nm）和16个近红外（780 nm）LED，用于测量样品PAR吸光系数。

• 成像面积：工作距离18.5cm，成像面积 10×13 cm；工作距离22.5cm，成像面积11×15cm。

• 光强异质性：测量区域光强异质性小于±7％。

• 测量参数：Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等。

应用领域

• 光合作用研究：可以在完全相同的条件下同时对大量样品进行成像

• 植物病理学：病斑部位（包括肉眼不可见时）成像以及病斑扩散的时空动力学

• 植物胁迫生 理学：肉眼不可见胁迫损伤的早期检测

• 遗传育种：出苗后大规模快速筛选高光合/抗旱/抗热/抗冻/抗病等植株

• 突变株筛选：快速筛选模式植物的光合突变株、抗逆突变株、产氢微藻突变株等

• 微藻毒理学：不同毒物浓度多个重复的样品一次测完，软件自动计算YZ比率

• 分子生物学：宏观水平上检测样品的绿色荧光蛋白（GFP）荧光

• 其它多种扩展研究

成像参数

Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), ETR, Abs, NIR和Red等

突变株的快速筛选

MAXI-IMAGING-PAM特别适合对幼苗、愈伤组织、微藻等进行突变株的快速筛选，适合于与光合突变株、抗逆（抗旱、抗盐、抗病等）突变株、产油/氢突变株等的快速筛选。

国外利用MAXI-IMAGING-PAM筛选突变株的典型客户如拜耳、BASF、孟山都、先正达等大型跨国农业巨头，以及各大农业育种、植物分子生物学等科研单位，例如澳大利亚植物功能基因组中心（阿德雷德大学）、德国尤里希表型植物表型研究中心（Julich Plant Phenotyping Centre）等等。

国内约一半的MAXI-IMAGING-PAM客户在进行突变株快速筛选工作，主要分布于中国科学院、中国农科院和各大高校。

突变株筛选实例一：国内某客户筛选的拟南芥突变株

突变株筛选实例二：产油突变株的筛选。Ajjawi et al, 2010, Plant Physiol., 152: 529-540.

突变株筛选实例三：光合突变株的筛选。Armbruster et al., 2010, Plant Cell, 22: 3439-3460.

调制叶绿素荧光成像实例

荧光成像与CO2气体交换的同步测量

（GFS-3000/IM-MAXI）：MAXI-探头与GFS-3000联用，在10 cm x 13 cm的面积上同步测量气体交换与荧光成像。

产地：德国WALZ

代表文献

数据来源：光合作用文献Endnote数据库

原始数据来源：Google Scholar

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