描述 Thermo Scientific Pierce Protein A Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.
These plates are uniformly and stably coated with Protein A, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.
Features of the plates:
• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)
Protein A Coated Microplates:
• Binds strongly to IgG from human, rabbit, guinea pig, pig, dog and rhesus monkey
• Binds strongly to mouse IgG2a, IgG2b and IgG3
• Binds to Fc region of antibodies for optimal orientation
Related Products
Pierce™ Protein A Coated Plates, Clear, 8-Well Strip
Pierce™ Protein A Coated Plates, White, 96-Well
Pierce™ Protein A Coated Plates, Black, 96-Well
For Research Use Only. Not for use in diagnostic procedures.
图表
Greater signal compared to directly coated antibody
Comparison of signal obtained by antibody bound to Protein A and Protein G coated plates, and antibody passively bound to polystyrene plates. Goat Anti-Biotin was serially diluted across Protein A, Protein G and polystyrene plates. The Goat Anti-Biotin was detected by the addition of biotin, followed by streptavidin-HRP and finally TMB substrate." data-omni-action="View figure"> 
Greater signal compared to directly coated antibody
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