裂解－GC/MS分析DNA

使用裂解器－色质联用分析去氧核糖和ＤＮＡ
Biosystem components, such as DNA, do not
normally lend themselves to thermal analysis.
Matrix complexity as well as lack of volatility
would seemingly rule out pyrolysis-GC/MS as the
method of choice. However, work has been
done that clearly shows thermal analysis can be
applied successfully to a biosystem component
like DNA.
Pyrolysis is a technique that thermally fragments
compounds and liberates volatile organic materials
from a matrix that is relatively non-volatile.
Sample analysis amounts are <100 mcg.
The volatiles are then separated by gas chromatography
and identified by use of a mass
selective detector in conjunction with a database
library like Wiley or Nist. CDS热裂解仪