采用 6545 Q-TOF LC/MS 系统进行代谢组学研究

Metabolomics allows researchers to
interrogate thousands of potential
biomarkers simultaneously, without a
priori knowledge of the underlying biology
or pathophysiology. Due to the complexity
of the samples, people need outstanding
mass resolution, mass accuracy, and
sensitivity as well as sophisticated data
analysis software. Agilent 6545 Q
-
TOF
LC/MS system and Agilent MassHunter
software are the
perfect combination to
fulfill the requirements.
In this application, serum extracts from
healthy subjects and
samples
with chronic
kidney disease (CKD) at stage 3 and
stage 5 are analyzed with 6545 LC/Q
-
TOF
and 1290 Infinity LC system. The mass
s
pectrometer is tuned using the novel
Swarm Autotune for optimal performance
in the m/z range of the metabolites.
Data was acquired in both positive and
negative ionization modes with reference
mass correction. The typical metabolomics
workflow is ill
ustrated here.
Big sensitivity gains improve results
The improved sensitivity of the 6545
Q
-
TOF LC/MS enables us to extract
5,817 features as opposed to 2,102
features on the previous generation of
Q
-
TOF under the same column load in
positive ionization mode. Even with a
five
-
fold reduction of column load, we
are still able to extract 2,489 features. Agilent 6545 四极杆飞行时间液质联用系统