CD 三氟乙醇产生的伴刀豆球蛋白A的伸展
The Stopped Flow method is used to analyze reactions occurred during the duration of several milliseconds
and several seconds meanwhile the CD measurement provides valuable information regarding the proteins
secondary structures and environment of aromatic amino acids. Therefore, CD measurement with Stoppedflow
method provides one of the best techniques in analyzing the proteins Unfolding and Refolding process.
Concanavalin A (derived from Jack bean) in natural state has abundant -sheet structures, however, it is
known that its structure changes into the one with rich -helix under trifluoroethanol (TFE) and its Unfolding
process has been reported also.
In this application, the model SFS-492 High-Speed Stopped-flow System was used to measure the Unfoldin JASCO圆二色光谱仪CD J-1500
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