Goat Anti-Dog Tumor necrosis factor

Goat Anti-Dog Tumor necrosis factor

Storage: 2 - 8 °C Package size: 96 determinations

PRINCIPLE OF THE METHOD
The TNF- kit is a solid phase phase sandwich enzyme linked immuno sorbent assay(ELISA). Samples , including standards of known TNF- concentrations and unknowns are pipetted into these wells. During the first incubation, the TNF- antigen and a biotinylated monoclonal antibody specific for TNF- are simultaneously incubated. After washing, the enzyme(streptavidin-peroxydase)is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TNF- present in the samples.