采用SPEX冷冻研磨机进行研磨-样品蛋白质（鱼）

• ELSEVIER ScienceDirect
www.elsevier.com/locate/yabio
Analytical Biochemistry 368 (2007) 3338


An assay for thiaminase I in complex biological samples


重组生物样品中硫胺酶I的活性检测-美国康奈尔大学
本实验所涉及的样品（鱼）经干燥后，在SPEX 6850冷冻研磨机内进行研磨，所得粉末即用于酶的提取．


Jeremiah W. Hanes a, Clifford E. Kraft b, Tadhg P. Begley a,*


a Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA


b Department of Natural Resources, Cornell University, Ithaca, NY 14853, USA
R
eceived 8 November 2006
Available online 7 June 2007


Abstract
An alternative method for measuring thiaminase I activity in complex samples is described. This assay is based on the selective consumption
of the highly chromophoric 4-nitrothiophenolate by thiaminase I, resulting in a large decrease in absorbance at 411 nm. This
new assay is simple and sensitive, and it requires only readily available chemicals and a visible region spectrophotometer. In addition, the
assay is optimized for high-throughput analysis in a 96-well format with complex biological samples.
Published by Elsevier Inc. SPEX6870 全自动冷冻研磨机 SPEX6970EFM 冷冻研磨机