Quantitative Analysis of Pyoluteorin in Anti-fungal

This paper investigated potential utility of capillary zone electrophoresis (CZE) for very succinct but robust quantitative analysis of pyoluteorin (Plt) in anti-fungal fermentation liquor of Pseudomonas species. The experimental conditions for the separation and quantification of Plt were optimized at first. The optimized conditions are: 80 mmol/L pH 8.40 Gly-NaOH buffer, 51 cm total length (42 cm effective) and 75 mm i.d. capillary, 230 nm wavelength, 25 kV, 13 mbar 10 sec pressure sample injection and 24oC air-coo领. Under the optimized conditions, the migration times of Plt and the internal standard phenobarbital are 2.09 and 2.49 minute, respectively, the linear response of Plt concentration ranges from 5.0 mg/mL to 1000 g/mL with high correlation coefficient (r = 0.99977, n = 9), the limits of detection (LOD) and quantification (LOQ) for Plt are 0.66 g/mL and 2.2 mg/mL, the precision values (expressed as RSD) of intra-day and inter-day are 1.19-1.94% and 1.55-6.21%, respectively, the recoveries of Plt at three concentration levels of 750, 250 and 50 mg/ml range from 90.31%, to 97.85% and to 98.96%, respectively. The developed method can be well used for the quantification of Plt in the fermentation liquor. GX毛细管电泳仪(紫外检测)CL1030