TSK-GEL SWXL系列色谱柱(尺寸排阻色谱法)对标准蛋白混合样品的分析

色谱柱 : TSK-GEL SWXL series
流动相 :20mmol/L phosphate buffer + 0.3mol/L NaCl (pH7.0)
流速 : 1.0mL/min
检测 : UV (280nm)
温度 : 25C
进样体积 : 10L
样品 : Standard sample
液相色谱仪 : Agilent 1120 Compact LC (gradient auto system)
样品组成 : 1. Thyroglobulin (M.W.:640KDa, bovine thyroid, Sigma T1001, 3g/L)
2. Mouse monoclonal antibody (M.W.:150KDa, in house, IgG1,2g/L)
3. Conalbumin (M.W.:47KDa, Wako pure chemicals, Japan, 016-17073, 4g/L)
4. Ribonuclease A (M.W.:13.7KDa, Sigma R4875, 4g/L)
5. Vitamin B12 (M.W.:1355, Wako pure chemicals, Japan, 224-00344, 0.2g