TSK-GEL SuperSW系列色谱柱(尺寸排阻色谱法)对标准蛋白

色谱柱 : TSKgel SuperSW series
流动相 : 20mmol/L Phosphate buffer + 0.3mol/L NaCl (pH7.0)
样品 : MW-MARKER PROTEINS (HPLC), cat:46804000, ORIENTAL YEAST CO.,LTD, Japan
流速 : 0.35mL/min
检测 : UV (280nm)
温度 : 25C
进样体积 : 2L
液相色谱仪 : Agilent 1120 Compact LC (gradient auto system)
样品组成 :
1. Glutamate dehydrogenase (yeast, M.W.:290KDa, 1.83g/L)
2. Lactate dehydrogenase (pig heart, M.W.:142KDa, 1.58g/L)
3. Enolase (yeast, M.W.:67KDa, 1.35g/L)
4. Myokinase (yeast, M.W.:32KDa, 1.31g/L)
5. Cytochrome C (horse heart, M.W.:12.4KDa, 1.25g/L)